Preimplantation Development of Tetraploid Mouse Embryo Produced by Cytochalasin B.
نویسندگان
چکیده
منابع مشابه
Development of cytochalasin in B-induced tetraploid and diploid/tetraploid mosaic mouse embryos.
By subjecting ¥x (CBA x C57/BL) x A eggs at the time of 2nd cleavage to 10 /tg/ml of cytochalasin B (CB), tetraploidy was produced in 52 % of 2-cell eggs and 35 % of 3-cell eggs. 2n/4n mosaic embryos were produced from 2-, 3and 4-cell eggs and amounted to 20 % of all treated eggs. 80 % of tetraploid embryos developed in vitro into regular blastocysts with half the cell number of control diploid...
متن کاملDevelopment of cytochalasin B-induced tetraploid and diploid/tetraploid mosaic mouse embryos
By subjecting ¥x (CBA x C57/BL) x A eggs at the time of 2nd cleavage to 10 /tg/ml of cytochalasin B (CB), tetraploidy was produced in 52 % of 2-cell eggs and 35 % of 3-cell eggs. 2n/4n mosaic embryos were produced from 2-, 3and 4-cell eggs and amounted to 20 % of all treated eggs. 80 % of tetraploid embryos developed in vitro into regular blastocysts with half the cell number of control diploid...
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Despite the fact that a variety of experimental techniques have been devised over the years to induce tetraploid mammalian embryonic development, success rates to date have been limited. Apart from the early study by Snow, who obtained development to term of a limited number of cytochalasin B-induced tetraploid mouse embryos, no other researchers have achieved development of tetraploid embryos ...
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SUMMARY Since at oocyte retrieval for in vitro fertilization there is some FF become incorporate to egg, we decided to investigate the effect of this fluid on preimplantation embryo development in vitro. FF was obtained at the time of oocyte retrieval for IVF, centrifuged and then either inactivated by heat and stored at 4°c or without inactivation stored at-20°c until used. Late 2-cell emb...
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ژورنال
عنوان ژورنال: Experimental Animals
سال: 1995
ISSN: 1341-1357,1881-7122
DOI: 10.1538/expanim.44.105